Draft Agenda 

Day 1Day 2Day 3AbstractsBreakout Sessions
PDF of Full Agenda Here

Wednesday, January 29

Registration & Coffee 7:00 AM

Welcome 8:00 – 8:40 AM

Holly Aaron (University of California, Berkeley), Opening Remarks

Wolfgang Becker (Becker-Hickl, GmbH), Introduction to time-correlated fluorescence lifetime imaging (TCSPC FLIM)

                 Short Break 8:40 – 9:00 AM               

Fluorescent Lifetime Imaging I (Chair: Wolfgang Becker) 9:00 – 10:15 AM

Horst Wallrabe (University of Virginia), Autofluorescence – multiexponential or incomplete…..that’s the question

Vladislav Shcheslavskiy (Becker-Hickl, GmbH), Oxygen measurements by time-correlated single photon counting

Angelika Rück (University of Ulm), NADH/FAD/FMN-FLIM in bioenergetic alterations


VENDOR FAIR I                      10:15 – 11:15 AM

Metabolic investigations in clinical imaging (Chair: Hauke Studier)  11:15 AM – 12:30 PM

Ammasi Periasamy (Keck Center, University of Virginia), Investigation of metabolism in living prostate cancer cells: FLIM-FLIRR microscopy

Melissa Skala (University of Wisconsin, Madison), Metabolic imaging of immune cell function

Michael Roberts (University of Queensland Diamantina Institute), Using FLIM to characterise redox changes in liver injury and disease


LUNCH  12:30 – 2:00 PM


Neuroscience (Chair: Andrea Gomez, University of California, Berkeley)             2:00 – 3:40 PM

Evan Miller (University of California, Berkeley), Electrophysiology, unplugged: Fluorophores to measure voltage in living systems

Fangyuan Ding (California Institute of Technology), Constitutive splicing and economies of scale in gene expression

Saskia de Vries (Allen Brain Institute), A large-scale standardized calcium imaging pipeline reveals functional organization of the mouse visual cortex

Na Ji (University of California, Berkeley), Imaging neural activity at high spatiotemporal resolution


VENDOR FAIR II         3:45 – 5:00 PM

Or BREAKOUT SESSION   4:00 – 4:45PM  (Venetian Room)

Ariel Waldman (Global Director of Science Hack Day), Life under the ice


Innovations in Imaging I (Chair: Benjamin Smith, University of California, Berkeley)   5:00 – 6:15 PM

Meredith Calvert (Denali Therapeutics), Advanced imaging approaches in drug discovery: Exploring macromolecular biodistribution to brain targets

Aaron Streets (University of California, Berkeley), Imaging and sequencing single cells

Laura Waller (University of California, Berkeley), 3D phase microscopy with scattering samples


[7PM – Dinner on your own or Speaker Dinner for Speakers, Organizers and Chairs]

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Thursday, January 30

Coffee  7:30 AM

Developing Microscopy Tools for the Future (Chair: DeLaine Larsen, University of California, San Francisco)   8:00 – 9:40 AM

Mateo Sanchez-Lopez (Stanford University), Directed evolution of TEV protease and application to fluorescent reporters of Ca+2 activity and GPCR activity in cells

Kwanghun Chung (Massachusetts Institute of Technology), Towards holistic imaging and rapid phenotyping of complex biological systems

Xiaoyu Shi (University of California, Irvine), Label-retention expansion microcopy enables molecular-resolution imaging

Joshua Vaughan (University of Washington), Super-resolution microscopy made simple

           Break 9:40 – 10:00 AM

Fluorescence Lifetime Imaging II (Chair: Denise Schichnes, University of California, Berkeley)   10:00 – 10:50 AM

Emilie Steinmark (Kings College London), Combining fluorescent molecular rotors and time-resolved fluorescent anisotropy in viscosity imaging 

Alzbeta Chorvatova (International Laser Centre, Bratislava, Slovakia), Environmental sensing by time-resolved autofluorescence monitoring in algae

          Short break 10:50 – 11:00 AM

Innovations II (Chair: Blaise Ndjamen, Gladstone Institutes)   11:00 AM – 12:30 PM

Samantha Fore (Zeiss Microscopy), Multiplex mode for the LSM 9 series with Airyscan 2: Fast and gentle confocal super-resolution in large volumes

Alberto Diaspro (Nanoscopy, Istituto Italiano di Tecnologia, Genova, Italy), Multi-messenger optical microscopy coupling fluorescence and label-free imaging

Elizabeth Hillman (Columbia University), New developments and applications of SCAPE microscopy for fast 3D imaging

Sara Abrahamsson (University of California, Santa Cruz), M25 multifocus 25-plane microscope with camera array for neural imaging


LUNCH 12:15 – 1:30 PM


Investigating Mitochondrial Action (Chair: Samantha Lewis, University of California, Berkeley)   1:45 – 3:00 PM

John Albeck (University of California, Davis), Live-cell analysis of ATP homeostasis in single cells

Laura Newman (Salk Institute), Mitochondrial/ER contacts modulate release of mtDNA from mitochondria

Natalie Porat-Shliom (National Cancer Institute, National Institutes of Health), Mitochondrial dynamics in intact tissues using intravital microscopy


VENDOR FAIR III   3:00 – 4:45 PM

Or BREAKOUT SESSION   3:15 – 4:30 PM   (Venetian Room)

Gender-inclusion in microscopy

(Chairs: Meredith Calvert and Gisele Giorgi, BioImaging North America Diversity Working Group, Abby Dernburg, UC Berkeley)


Computation & Analysis I (Chair: Deepa Sridharan)     4:45 – 6:00 PM         

Kevin Eliceiri (University of Wisconsin, Madison), The ImageJ ecosystem: An open and extensible platform for biomedical image analysis

Beth Cimini (Broad Institute), Getting the most data from your images with CellProfiler and morphological profiling

Loic Royer (CZ Biohub), noise2self and napari — novel tools to enhance and visualize microscopy data

POSTER SESSION & RECEPTION   6:00 – 8:30 PM    (Venetian Room)

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Friday, January 31

Coffee  8:00 AM

Microscopy in a Clinical Setting (Chair: Annette Chan, San Francisco State University)   8:30 – 9:55 AM

Wolfgang Becker (Becker-Hickl), Cancer detection by NADH/FAD FLIM           

Yousuf Mohammed (University of Queensland Diamantina Institute), Imaging topical drug pharmacokinetics in human skin – Opportunities and challenges

Marina Shirmanova (Privolzhsky Research Medical University, Russia), What FLIM of NAD(P)H reveals about tumor metabolism          


ON- AND OFF-SITE DEMOS  10:15 AM – 12:15 PM

See Demo Info Here

TUTORIALS  10:15 – 12:30

10:15 – 11:00 AM Fluorescence Lifetime Imaging Analysis – Wolfgang Becker

11:00 – 11:45 AM FIJI is Just ImageJ – Kevin Eliceiri

11:45 AM – 12:30 PM Introduction to CellProfiler – Beth Cimini                         


LUNCH    12:30 – 1:30 PM


POSTER WINNER Announcement     2:00 – 2:10 PM

Industry Innovations (Chair: Eva Nichols)    2:10 – 2:45 PM

Steven Smith (Refined Optical Systems), TBA

Jim Sims (Hamamatsu), TBA

TBA (ThorLabs), Bessel beam

Byron Hartman (Miltenyi BioTec), Exploring the architecture of biology with tissue clearing and light sheet microscopy

Short Break 2:45 – 3:00

Subcellular Imaging (Chair: Julia Lazzari-Dean, University of California, San Francisco)       3:00 – 4:15 PM

Thomas Gensch (Cellular Biophysics, Forschungszentrum Jülich), Fluorescence lifetime imaging of intracellular sodium concentration

Abby Dernburg (University of California, Berkeley), Measuring diffusion within a subcellular compartment

Steven Vogel (NIAAA/NIH), Using FRET spectroscopy and FCS to study conformational changes associated with Venus-tagged CaMKII activation and T-site interactions

                     Dessert break  4:15 – 4:45 PM  

Computation (Advanced Bioimaging Center Edition, Chair: Gokul Upadhyayula & Eric Betzig)    4:45 – 6:30 PM

Yair Rivenson ((University of California, Los Angeles), Deep learning enhanced fluorescence microscopic imaging

Aydogan Ozcan (University of California, Los Angeles), Deep learning-enabled computational microscopy and sensing

Nicholas Sofroniew (Chan Zuckerberg Initative), napari: A multi-dimensional image viewer for python

Kristen Kwan (University of Utah), Computational approaches to dissecting developmental tissue morphogenesis




Start times each day will be 8:00AM and most talks will end by ~6:30pm. Receptions and poster sessions will begin at the end of talks.



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