-Lightsheet configuration
-Cleared sample imaging
-Objectives: 20X CLARITY objective, 20X Water, 5X CLARITY
-Excitation lasers: 405nm, 440nm, 488nm, 514nm, 561nm, 642nm
-Live imaging: Possible, but use Serenity instead which is water-based only
-Post-processing software: Arivis on Morpheus’ workstation
Laser Details:
Laser 405nm 50mW
Laser 445nm 40mW
Laser 488nm 50mW
Laser 515nm 50mW
Laser 561nm 50mW
Laser 638nm 75mW
Laser Blocking Filters and Filtersets:
Filter Module LBF 445/515/561
Filter Module LBF 405/488/561/638
Filters for Two-Detector Modules:
Filter Module Lightsheet Z.1 — GFP-Cy3
SECONDARY FILTER TURRET:
Filter Module Lightsheet Z.1 – CFP-YFP
Filter Module LSFM – CFP-mCherry
Filter Module LSFM – YFP-mCherry
Zeiss video on mounting a sample in an agarose-filled capillary
