Training

5 easy steps to get trained on most* of the MIC instruments

1.  Attend the Microscopy Training Lecture (1.5 hours), offered once a month, schedule HERE. (RSVP HERE.)
2.  Fill out the MIC User Registration Form HERE. (Describe your samples & imaging needs.)
3.  Sign-up for an iLabs account HERE. (Your lab manager or PI will need to add you to their lab.)
4.  Read the MIC_Rules & Regulations, to which all users must adhere. By gaining access and using the equipment at the MIC, you are agreeing to abide by these rules. 
5.  Once you have your sample ready, send an email to Holly and Feather to set up a date for hands-on training with your sample (most instruments ~2 hours). 

*Note: some instruments require additional training, such as EH&S Laser Safety Training, or FLIM training, while a select few require shorter training sessions at reduced or no cost. Filling out the user form will help us determine what kind of training you’ll need.

Frequently Asked Questions:

Who may use the Molecular Imaging Center?
The Molecular Imaging Center is open to all scientists from within the University and outside. Outside users are charged a greater recharge rate to use the facility, generally 2X the UCB rate. ALL users must be trained before accessing the equipment. In rare cases, we can do imaging for you (for a surcharge). 

What should I know before coming to the MIC?
You should know what your goals are for imaging. Are you looking for just a pretty picture, or do you want to do longterm time-lapse imaging, or quantification of proteins, or tracking of particles? You should know what dyes/fluorophores you are using and what excitation and emission peaks those dyes have. You should know how to prepare your samples for imaging. You should know if your samples are live or fixed and whether or not they are biohazardous. We are happy to consult with you about your imaging goals and requirements at any time.

How much is training?
Standard training costs $250 per person and includes lecture, hands-on training with your sample, and additional trainings as needed on the same or similar instrument. Outside users are charged $500 for training. Some instrument training fees are reduced or slightly increased, so please see our instruments spreadsheet or iLabs for the most up-to-date information on training costs. 

Do I need to attend the training lecture? And why?
Yes, everyone must attend the training lecture. The lecture is meant to give everyone a basic understanding of fluorescence imaging and laser-based confocal imaging, as well as to share tips for successful imaging early on in the microscopy data collection process. We will also give an overview of the resources available at the MIC and can answer any microscopy questions you may have. Additionally, we want to meet our users and get to know what your imaging goals are. 

What should I bring to training? Bring a typical sample that you plan to image.  For fixed samples, make sure to use glass slides with number 1.5 coverslips that are sealed, dry, and clean.  We do not recommend training on live samples, as we want to focus on instrument settings and setup and not the health of the precious sample. If you can bring a fixed sample that is similar to your live sample, that is ideal. Or if you must bring a live sample, for an inverted systems, please use a coverglass-bottom dish (again, #1.5 coverslip), such as those available from MatTek or ibidi, and please use culture media without phenol-red or riboflavin to reduce background fluorescence. For upright instruments, please check in, as the clearance and depth of focus varies depending on the instrument. For lightsheet, please email us for the sample prep guide! Don’t hesitate to ask if you have any questions regarding sample prep.

What happens during training? 
You will receive one-on-one hands-on training with your sample on the most appropriate microscope system for your experiment. You will be taught how to start up and shut down the system safely. On select systems, you may also be trained on laser alignment (1-3 hours typical, more with laser training).  If you have specific expectations or goals, please let us know ahead of time.

What happens after training?
You will receive calendar access to schedule the instrument on iLabs, as well as the necessary door-codes or building access (forms & signatures required for LKS and LSA; after-hours access only available to UCB graduate students & postdocs.).  Additional training/guidance is available after the initial session on an as-needed basis and is non-billable (i.e. FREE).

How much does it cost to use MIC instruments?
Hourly charges on most MIC instruments range from $8-46/hour for campus users.  All prices are listed on iLabs. Outside user rates are generally 2x, unless posted elsewhere on iLabs. 

What other services does the MIC offer?
(1) We offer a slide scanning service. 

(2) We can training you on your own instrument at $250 for the first 2 hours and $65/hr for each additional hour.  

(3) We are also available for consultation regarding all matters microscopy. 

(4) We can also help write or edit your Methods Section for your publication.

(5) We are happy to write letters of support for grants. 

How do I get trained for laser safety?
If you need laser safety training (check with Holly), you’ll need to:

I used one of the MIC’s instruments to obtain data for a publication.  How should I acknowledge that information in my manuscript?
Congratulations on your paper and thank you for remembering us!  Please consult the Acknowledgments page for grant and citation information.  When your paper is in press, contact Feather so we can feature it on our MIC Publications page!

 

Have additional questions? Please ask – email Feather or Holly or setup a time to come chat.