Training

5 easy steps to get trained on most* of the MIC instruments

1.  Attend the Microscopy Training Lecture (1.5 hours), offered once a month, schedule HERE. (RSVP HERE.)
2.  Fill out the MIC User Registration Form HERE. (Describe your samples & imaging needs.)
3.  Sign-up for an iLabs account HERE. (Your lab manager or PI will need to add you to their lab.)
4.  Read the MIC_Rules & Regulations, to which all users must adhere. By gaining access and using the equipment at the MIC, you are agreeing to abide by these rules. 
5.  Once you have your sample ready, send an email to Holly and Jen to set up a date for hands-on training (typically 2 hours). DO NOT request training through iLabs.

*Note: some instruments require additional training, such as EH&S Laser Safety Training, or FLIM training, while a select few require shorter training sessions at reduced or no cost. Filling out the user form will help us determine what kind of training you’ll need.

Frequently Asked Questions:

Who may use the Molecular Imaging Center?
The Molecular Imaging Center is open to all scientists, from within the University and outside. Outside users are charged a greater recharge rate to use the facility. ALL users must be trained before accessing the equipment. In some cases, we can do imaging for you (at a greater cost). 

What should I know before coming to the MIC?
You should know what your goals are for imaging. Are you looking for just a pretty picture, or do you want to do longterm time-lapse imaging, or quantification of proteins, or tracking of particles? You should know what dyes/fluorophores you are using and what excitation and emission peaks those dyes have. You should know how to prepare your samples for imaging. You should know if your samples are live or fixed and whether or not they are biohazardous. We are happy to consult with you about your imaging goals and requirements at any time.

How much is training?
It varies, depending on the system you want to use. Please see our instruments spreadsheet or iLabs for the most up-to-date information.

What should I bring to training?
Bring a typical sample that you will be imaging.  For fixed samples, make sure to use glass slides with number 1.5 coverslips that are sealed, dry, and clean.  For live samples on inverted systems, please use a glass-bottom dish.  Please use culture media without phenol-red or riboflavin to reduce background fluorescence. Don’t hesitate to ask if you have any questions regarding sample prep.

What happens during training? 
You will receive one-on-one hands-on training with your sample on the most appropriate microscope system for your experiment. You will be taught how to start up and shut down the system safely. On select systems, you may also be trained on laser alignment (1-3 hours typical, more with laser training).  If you have specific expectations or goals, please let us know ahead of time.

What happens after training?
You will receive access to schedule equipment on iLabs, as well as the necessary doorcodes or building access.  Additional training/guidance is available after the initial session on an as-needed basis and is non-billable (i.e. FREE).

How much does it cost to use MIC instruments?
Hourly charges on most MIC instruments range from $7-45/hour for campus users.  All prices are listed on iLabs.

What other services does the MIC offer?
We offer a slide scanning service. 

We can training you on your own instrument at $250 for the first 2 hours and $65/hr for each additional hour.  

We are also available for consultation regarding all matters imaging-related. 

How do I get trained for laser safety?
If you need laser safety training (as determined by Holly or Jen), you’ll need to:

I used one of the MIC’s instruments to obtain data for a publication.  How should I acknowledge that information in my manuscript?
Congratulations on your paper!  Please consult the Acknowledgments page for grant and citation information.  When your paper is in press, contact Jen so we can feature it on our MIC Publications page!

 

Have additional questions? Please ask – email  Jen or Holly or setup a time to come chat.