Zeiss LSM980 (K)

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Our newest instrument, installed in 2020, the Zeiss Axio Observer inverted laser scanning confocal microscopy, LSM980 with Airyscan 2, Spectra-Physics MaiTai HP laser, and Becker-Hickl TCSPC FLIM system has the following features:

• 3-channel LSM with GaAsP detectors and spectral detection
• Airyscan 2 with Multiplex Mode for super-resolution and/or sensitive live cell imaging
• 1-photon lasers for confocal/Airyscan: 405, 488, 514, 561, and 639nm
• 2-photon: SP Maitai HP 690 – 1040nm
• Becker-Hickl Time-Correlated Single Photon Counting (TCSPC) fluorescence lifetime imaging system (FLIM) with:
  • BH SPC-150NX TCSPC card
  • FLIM excitation lasers: 445, 488, 562, and 640nm
  • 2-color detection with HPM-100-40 hybrid detectors (2)
• Fluorescence correlation spectroscopy (FCS)
• Live cell imaging with full dark incubation chamber (heating, humidity, CO2)
• Objectives:
  • 10x air
  • 20x air
  • 40x/1.3 oil
  • 63x/1.4 PlanApo oil
  • 40x/1.2 W corr for FCS
• Lasers for FLIM: 445, 488, 562, and 640nm
• FLIM detection: 2-channel with HPM-100-40 detectors

*Requires special training

Zeiss tutorial videos for LSM 800/900/980