Instrumentation & Fees

The Molecular Imaging Center offers the following instruments:

Laser Scanning Confocal MicroscopesMulti-Photon InstrumentsSpinning Disk Confocal MicroscopesSpecialized InstrumentsEpifluorescence
Zeiss LSM 880 NLO AxioExaminer with Airyscan Zeiss LSM 880 NLO AxioExaminer with Airyscan Andor/Nikon Spinning DiskZeiss Lightsheet.Z1Zeiss AxioZoom
Zeiss LSM 880 NLO AxioExaminer with OPO Zeiss LSM 880 NLO AxioExaminer with OPO 3i Upright Spinning Disk ConfocalEssen IncuCyte ZoomLeica M205 Fluorescent Stereoscope
Zeiss LSM 780 NLO AxioExaminerZeiss LSM 780 NLO AxioExaminerNikon PALM Super Resolution SystemZeiss Axio Scan.Z1Nikon Widefield Epifluorescence Microscope
ZeissLSM 710 AxioObserverZeiss LSM 510 META NLO AxioImager3i Upright Spinning Disk Confocal
Zeiss LSM 510 META NLO AxioImagerZeiss 510 NLO AxioVert with FLIM3i/Sutter In Vivo MOM/SLM
Zeiss 510 NLO AxioVert with FLIMZeiss 5-LIVE AxioSkop FS
Zeiss LSM 510 META AxioPlan

Below is a brief description of each instrument. Details, specifications, and usage fees can be found here. We also have 2 offline computer workstations for image processing. Instrument scheduling can be found on the MIC iLabs page. You can also check out the OpenMIC series, “Instrument Highlight” for detailed profiles of the instruments. Please contact us with any questions or specific imaging needs.

CONFOCAL & MULTI-PHOTON LASER SCANNING MICROSCOPES

TrinityZeiss LSM 880 NLO AxioExaminer with Airyscan (Trinity)

– Ultra-sensitive, upright confocal featuring 2-photon imaging and GaAsP detectors
– Airyscan super-resolution technology, up to 1.7-fold better resolution than standard scanning confocal microscopes
– Large motorized stage for combined electrophysiology and imaging
– 20X SCALE objective in addition to standard objectives

NeoZeiss LSM 880 NLO AxioExaminer with OPO (Neo)

– 2-photon imaging from 700-1300 nm
– Ultra-sensitive, upright confocal imaging
– Large motorized stage for combined electrophysiology and imaging

Rachel copyZeiss LSM 780 NLO AxioExaminer (Rachel)

– Ultra-sensitive, upright confocal featuring 2-photon imaging with GaAsP detectors
– Fully spectral system with imaging in the visible range from 400-700nm
– Large fixed stage for combined electrophysiology and imaging

Optimus copyZeiss LSM 710 AxioObserver (Optimus Prime)

– 34-Channel Spectral Detector
– Motorized XY-stage for tile scan and multi-position experiments
– Live cell imaging capabilities: full incubation chamber with temperature and CO2 control and a heated stage.

 

MF3i/Sutter In Vivo MOM-SLM (Millenium Falcon)

– Dual-multiphoton Movable Objective Microscope (MOM) system uniquely designed for in vivo optogenetic studies using a Spatial Light Modulator (SLM).
– Special training required.

Deckard copyZeiss 510 NLO AxioVert 200M MAITAI HP (Deckard)

– Spectra-Physics MaiTai HP laser for 2-photon imaging (690nm – 1020nm)
– Spectra-Physics Pulse Picker and Frequency Doubler (375nm – 500nm)
– Becker-Hickl SPC-830 Time-Correlated Single Photon Counting board for FLIM
– Features: Motorized stage, LCI Stage-top Chamide TC incubator for temperature, CO2, and humidity control and physiology triggering.

Pris copyZeiss LSM 510 META NLO AxioImager with MAITAI DEEPSEE (Pris)

– Upright confocal and multiphoton laser scanning microscope with a Spectra-Physics MaiTai HP DeepSee laser for 2-photon Imaging (700nm – 1020nm)
– Spectral emission profiling and separation (linear unmixing)
– Live cell imaging

Boomer copyZeiss LSM 510 META AxioPlan (Boomer)

– Upright laser scanning confocal for fixed, 3-color visible imaging: green, red, and far-red.

 

Eve copyZeiss 5-LIVE AxioSkop FS (Eve)

– Specialized upright, fixed-stage line-scanning confocal system for doing very fast imaging.
– Warner TC-324B Heater Controller and physiology triggering/set-up available.

Max copyAndor/Nikon Spinning Disk (Max)

– Inverted spinning disk confocal with a motorized XY-stage
– Full enclosure incubation chamber for humidity, CO2 and temperature control for live cell imaging.

Enterprise copy

3i SDC with SLM (Enterprise)

– Upright spinning disk confocal (SDC) microscope with Spatial Light Modulators (SLM)
– Large platform, Sutter-motorized XY-stage for electrophysiology
– Ideal for zebrafish, Drosophila, and brain slice work with 1-photon and 2-photon optical stimulation.

Megatron copyNikon PALM with AO and SDC (Megatron)

– Super-resolution system with unique laser control for TIRF-based localization microscopy and adaptive optics for z-position
– Additional features: spinning disk confocal, full incubation enclosure, and micro-injection set-up.

EPIFLUORESCENCE MICROSCOPES & SPECIALIZED INSTRUMENTS

Morpheus copyZeiss LightSheet.Z1 (Serenity & Morpheus)

– Selective Plane Illumination Microscopy (SPIM).
– Excellent for imaging small, transparent organisms, such as zebrafish and Drosophila.

 

BayMax copyZeiss Axio Scan.Z1 (Baymax)

– Automated slide scanner for brightfield and fluorescent slides.
– Slide scanning service available. Please see the information sheet for details.

 

 

IncuCyte copyEssen IncuCyte Zoom

– Fully automated imager inside an incubator, allowing for long-term (i.e., multi-day) imaging.
– Available optics: phase contrast, green fluorescence, and red fluorescence at 4x, 10x, or 20x.
– Features whole well imaging at 4x.

Zoom copyZeiss AxioZoom (Zoom)

– Macro scope with 16x motorized zoom, transmitted, and reflected light.
– Motorized XY-stage, ideal for multi-acquisition (i.e., multi-position and tiling).
– Fluorescent illumination with the following filter cubes: DAPI, eGFP, mCherry, eCFP, and eYFP.

Johnny5 copyLeica M205 Fluorescent Stereoscope (Johnny-5)

– Fluorescent stereoscope ideal for screening and dissections.
– Available filter cubes: CFP, GFP, YFP, and DS-Red.
– Not equipped with a camera.

Herzmark copyNikon Widefield Epifluorescent Microscope (The Herzmark)

– Inverted compound microscope with DIC optics.
– Features incubation chamber, motorized stage, and Nikon Perfect Focus System.