Instrumentation & Fees

Below is a brief description of each instrument. Details, specifications, and usage fees can be found here. We also have offline computer workstations for image processing. Instrument scheduling can be found on the MIC iLabs page. You can also check out the OpenMIC series, “Instrument Highlight” for detailed profiles of some of the instruments. Please contact us with any questions or specific imaging needs.

Our equipment falls into five general categories:

CONFOCAL & MULTI-PHOTON LASER SCANNING MICROSCOPES

TrinityZeiss LSM 880 NLO AxioExaminer with Airyscan and Coherent Chameleon Vision (Trinity)

– Ultra-sensitive, upright laser scanning confocal with 1-photon and 2-photon imaging, GaAsP detectors, 34-channel spectral imaging
– Airyscan super-resolution technology, up to 1.7-fold better resolution than standard scanning confocal microscopes
– Large motorized stage for combined electrophysiology and imaging
– 20X CLARITY objective in addition to standard objectives

NeoZeiss LSM 880 NLO AxioExaminer with Coherent Chameleon MPX OPO (Neo)

– Ultra-sensitive, upright confocal imaging with large motorized fixed stage
– 2-photon imaging from 700-1300 nm
– Visible lasers: 405, 458, 488, 514, 561, 591, 633nm

Rachel copyZeiss LSM 780 AxioExaminer with Spectra-Physics Maitai (Rachel)

– Ultra-sensitive, upright confocal featuring 2-photon imaging with GaAsP detectors
– 34-channel spectral system with imaging in the visible range from 400-700nm; visible lasers: 405, 488, 514, 561, 633nm
– Large fixed stage for combined electrophysiology and imaging

Optimus copyZeiss LSM 710 AxioObserver (Optimus Prime)

– Inverted laser scanning confocal with 34-channel spectral detection
– Motorized XY-stage for tile scan and multi-position experiments
– Live cell imaging capabilities: full incubation chamber with temperature and CO2 control and heated stage
– Lasers: 405, 458, 488, 514, 561, 591, 633nm

MF3i/Sutter In Vivo 2p Movable Objective Microscope (MOM) with 2p Phasor Spatial Light Modulator (Millenium Falcon)

– Dual-multiphoton Movable Objective Microscope (MOM) system uniquely designed for in vivo optogenetic studies using a Spatial Light Modulator (SLM) for stimulation at 1040nm with high-power femtoTrain laser
Special training required. 

Deckard copyZeiss 510 NLO AxioVert200M with Maitai HP and BH SPC-150 for FLIM (Deckard)

– Inverted laser scanning confocal with motorized stage for fluorescent lifetime imaging (FLIM)
– Spectra-Physics MaiTai HP laser for 2-photon (690nm – 1020nm)
– Spectra-Physics Pulse Picker and Frequency Doubler (375nm – 500nm)
Becker-Hickl SPC-150 Time-Correlated Single Photon Counting board for FLIM
– Live cell imaging: LCI Stage-top Chamide TC incubator for temperature, CO2, and humidity control
Special training required.

Pris copyZeiss LSM 510 META NLO AxioImager with Maitai DeePSee (Pris)

– Upright confocal and multiphoton laser scanning microscope with a Spectra-Physics MaiTai HP DeepSee laser for 2-photon Imaging (700nm – 1020nm)

Boomer copyZeiss LSM 510 META AxioPlan (Boomer)

– Upright laser scanning confocal for fixed, 3-color visible imaging: green, red, and far-red (excitation: 488, 543, and 633nm)

 

Eve copyZeiss 5-LIVE AxioSkop FS (Eve)

– Specialized upright, fixed-stage line-scanning confocal system for doing very fast imaging
– Warner TC-324B Heater Controller and physiology triggering/set-up available

 

SPINNING DISK CONFOCAL MICROSCOPES

Nikon Spinning Disk Confocal (Wall-E)

– Inverted Yokogawa spinning disk confocal microscope for live cell imaging
– Nikon PerfectFocus
– Lasers: 405, 488, 561, and 637nm
– Full incubation enclosure with CO2, humidity, and heating
– Motorized XY stage

Enterprise copy3i SDC with SLM (Enterprise)

– Upright spinning disk confocal microscope with Spatial Light Modulators (SLM)
– Large platform, Sutter-motorized XY-stage for electrophysiology
– Ideal for zebrafish, Drosophila, and brain slice work with 1-photon or 2-photon holographic stimulation

Max copyNikon/Andor Spinning Disk Confocal (Max)

– Inverted spinning disk confocal with a motorized XY-stage and Nikon PerfectFocus
– Full enclosure incubation chamber for humidity, CO2 and temperature control for live cell imaging.
– Andor Clara and Andor iXon cameras

LIGHTSHEET MICROSCOPES

Morpheus copyZeiss LightSheet.Z1 CLEARING (Morpheus)

– Selective Plane Illumination Microscopy (SPIM)
– Setup for cleared tissue imaging – CLARITY, CUBIC

 

Morpheus copyZeiss LightSheet.Z1 (Serenity)

– Selective Plane Illumination Microscopy (SPIM)
– Water-based samples only – e.g., zebrafish, tadpoles, Drosophila

 

EPIFLUORESCENCE MICROSCOPES

BayMax copyZeiss Axio Scan.Z1 (Baymax)

– 100-slide automated slide scanner for fluorescence or IHC
– Filter cubes for: DAPI, eGFP, Cy3, mPlum, Cy5
– Slide scanning service available. Please see the information sheet for details.

 

Herzmark copyNikon Widefield Epifluorescent Microscope (The Herzmark)

– Inverted compound microscope with DIC optics
– Incubation chamber, motorized stage, and Nikon Perfect Focus System
– Filters: DAPI, eGFP, Rhodamine, Cy5 (?)

IncuCyte copyEssen IncuCyte Zoom

– Fully automated imaging system inside a CO2 incubator, allowing for long-term (i.e., multi-day) imaging
– Phase contrast, eGFP, TexasRed; 4x, 10x, or 20x
– Features whole well imaging at 4x

Zoom copyZeiss AxioZoom v16 FL (Zoom)

– Macro scope with 16x motorized zoom, fluorescence, BF transmitted or reflected light
– Motorized XY-stage, ideal for multi-position or tiling
– Fluorescent illumination with the following filter cubes: DAPI, eCFP, eGFP, eYFP,  and mCherry

Johnny5 copyLeica M205 Fluorescent Stereoscope (Johnny-5)

– Fluorescent stereoscope ideal for screening and dissections
– Filter cubes: CFP, GFP, YFP, and DS-Red
Not equipped with a camera – for screening only.
 

COMPUTER WORKSTATIONS

DeepThought

– Windows workstation with GPU processor and SSD hard drives for faster processing and rendering
– Software: Bitplane Imaris, Zeiss Zen, FIJI, Matlab, Adobe Suite

Morpheus Processing Workstation(s)

– Windows workstation with GPU processor for faster processing and rendering
– Software: arivis Vision4D, Zeiss Zen, FIJI

 

Serenity Processing Workstation

– Windows workstation processing lightsheet data
– Software: Zeiss Zen, FIJI

 

Hal-9000

– MacPro for offline analysis
– Matlab, FIJI, Adobe Suite, Microsoft Office