Instrumentation & Fees

The CRL Flow Cytometry Facility operates in two locations: 491 Life Science Addition (LSA) and 461 Li Ka Shing (LKS) Building. There are 2 sorters and 4 analyzers in LSA; and there is 1 sorter and 1 analyzer in LKS.

Fees

Sorting is performed only by staff and is charged at:

Analysis is performed by users. Users must be trained prior to the use of the analyzers. Rates for use of the analyzers are as follows

Signing up for sorting or analysis or training

Reservations are handled by an online system called iLab. Users must obtain an iLab account before signing up to use the sorters or analyzers.

Once you have an iLab account, you can log in to iLab in order to request time for sorting or training. Requests for sorting or training must be approved by staff.
Once you are trained you can use iLab to sign up to use the analyzers.

Instruments in 491 LSA

The flow core located in room 491 Life Sciences Addition building (LSA) is managed by Hector Nolla. This core houses two cell sorters: a BD Influx (LSA) and a Beckman-Coulter Moflo. In addition, the LSA facility houses four analyzers: BD LSR II, BD LSR Fortessa, BD LSR Fortessa X20, and a Beckman-Coulter FC500.

Alma Valeros and Hector Holla operate the cell sorters. They also assist users with the analyzers.

Hours of operation are from 9:30AM -8:30PM, Monday to Fridays.
These are the only hours during which sorting can be performed. Trained users can access the analyzers 7 days a week, 24 hours a day.

You can call the facility: (510) 642-2843.

Sorters in LSA

Influx Cell Sorter (491 LSA)

Laser Parameter Filters Fluorochromes
488 nm FL1
FL2
550 LP | 530/40 BP
550 LP | 710/40 BP
FITC, GFP, YFP, CFSE
PerCP Cy 5.5, PI
405 nm FL8
FL9
480 LP | 460/50 BP
480 LP | 550/50 BP
Pacific Blue
Pacific Orange, AmCyan
UV FL6
FL7
550 LP | 460/50 BP
550 LP | 670/30 BP
Hoechst, DHPI
Red Hoechst
561 nm FL3
FL3
FL5
645 LP | 593/40 BP
700 LP | 670/30 BP
700 LP | 750 LP
PE, M-Cherry, RFP
PECy5
PECy7
640 nm FL10
FL3
FL11
700 LP | 660/20 BP
700 LP | 50 LP
APC
APC-Cy7

MoFlo Sorter (491 LSA)

Laser Parameter Filters Fluorochromes
488 nm FL1
FL2
FL3
FL4
505 DSP | 530/40 BP
555 DLP | 580/30 BP
710 DLP | 748/30 BP
710 DLP | 670/40 BP
FITC, GFP, YFP, CFSE
PE, PI
PECy7
PECy5, PerCP
648 nm
or
568 nm
FL7
FL7
655 LP | 670/30 BP
z
630/30 BP
APC
M-Cherry

Analyzers in LSA

LSR Fortessa X20 Analyzer (2013) (491 LSA)

Laser Parameter Name Filters
488 nm (Blue) PerCP-Cy5.5
FITC
685 LP | 710/50
505 LP | 530/30
405mm (Violet) BV 785
BV 711
BV 650
BV 605
AmCyan
Pacific Blue
750LP | 780/60
690LP | 710/50
635 LP | 670/30
595LP | 610/20
495 LP | 525/50
450/50
561 mm (Yellow- Green) PE-Cy7
PE-Cy5
PE-Texas Red
PE
750 LP | 780/60
635 LP | 670/30
595 LP | 610/20
570 LP | 586/15
640mm (Red) APC-Cy7
APC-Alexa700
APC
750 LP | 780/60
730 LP | 730/45
655 LP | 670/14

LSR Fortessa Analyzer (2010) (491 LSA)

Laser Parameter Name Filters
405nm (Violet) Pacific Blue
AmCyan
Qdot605
No Dichroic | 450/50
505LP | 525/50
595LP | 610/20
488mm(Blue) FITC
PerCP
505LP | 525/50
685LP | 710/50
561 mm(Yellow-Green) PE
PE-TexRed
PECy5
PECy7
No Dichroic | 582/15
600LP | 610/20
635LP | 670/30
750LP | 780/60
640mm (Red) APC
AlexaFluor700
APC-Cy7
No Dichroic | 670/30
690LP | 720/30
750LP | 780/60

LSR II Analyzer (2007) (491 LSA)

Laser Parameter Name Filters
488 Blue PE-Cy7
PerCP
PE
FITC
SSC (Side Scatter)
735 Dichroic LP / 780 -60
685 Dichroic LP / 695 -40
550 Dichroic LP / 575 -25
505 Dichroic LP / 530 -30
No Dichoic / 488 -10
405 Violet DAPI
Pacific Blue
505 Dichroic LP / 525 -50
No Dichroic / 450 -50
633 Red APC-Cy7
APC
735 Dichroic LP / 780 -60
No Dichroic / 660 -20

 

Instruments in 461 LKS

The Li Ka Shing (LKS) flow core is located in room 461. This core is managed by Kartoosh Heydari. It houses a BD Influx cell sorter and a BD LSR Fortessa analyzer.

Kartoosh operates the cell sorter and assists users with the analyzer. This core is part of the CIRM Institute at UC Berkeley.

Hours of operation are from 9:30AM -5:00PM, Monday to Fridays.
These are the only hours during which sorting can be performed in this facility. The analyzers are available for use 24h a day, 7 days a week.

You can contact the facility by calling (510) 664-4308 (Lab) or (510) 664-4307 (Office)

Sorter in LKS

Influx Cell Sorter (2013) (LKS 461)

Laser Parameter Filters Fluorochromes
488 nm FL1
FL2
550 LP | 530/40 BP
550 LP | 710/40 BP
FITC, GFP, YFP, CFSE
PerCP Cy 5.5, PI
405 nm FL8
FL9
480 LP | 460/50 BP
480 LP | 550/50 BP
Pacific Blue
Pacific Orange, AmCyan
UV FL6
FL7
550 LP | 460/50 BP
550 LP | 670/30 BP
Hoechst, DHPI
Red Hoechst
561 nm FL3
FL3
FL5
645 LP | 593/40 BP
700 LP | 670/30 BP
700 LP | 750 LP
PE, M-Cherry, RFP
PECy5
PECy7
640 nm FL10
FL11
700 LP | 660/20 BP
700 LP | 50 LP
APC
APC-Cy7

LSR Fortessa Analyzer (2010) (491 LSA)

Laser Parameter Name Filters
405nm (Violet) Pacific Blue
AmCyan
Qdot605
No Dichroic | 450/50
505LP | 530/50
595LP | 610/20
488mm(Blue) FITC
PerCP
505LP | 525/50
685LP | 710/40
561 mm(Yellow-Green) PE
PE-TexRed
PECy5
PECy5.5
PECy7
No Dichroic | 585/15
600LP | 610/20
635LP | 660/20
| 712/21
750LP | 780/60
640mm (Red) APC
AlexaFluor700
APC-Cy7
No Dichroic | 670/14
690LP | 730/45
750LP | 780/60
UV Laser Indo-1 (Blue)
Indo-1 (Violet)
495 LP | 525/50
No Dichroic | 450/50

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